Biology 110 - Cell Biology Fall 2009

Media Theater M110 - Tuesday and Thursday, 2:00 - 3:45 PM

Lindsay Hinck

351 Sinsheimer, Office, 459-5253

324 Sinsheimer, Lab, 459-2546

Office Hours - Wednesday 1:30-3:30 in Sinsheimer rm 401

Email address:  hinck@biology.ucsc.edu  I answer class emails on Friday morning

Course Web Site – http://bio.classes.ucsc.edu/bio110/

Podcasts of lectures available at:   https://media4.ucsc.edu/webcast/

Text: Alberts et al. – Molecular Biology of the Cell,  5th  Edition, 2008

QuickTime conversions for animations for those of you who have the new version of Quicktime

http://www.garlandscience.com/textbooks/0815341059.asp?type=resources

 

 

Date

Topic

Chapters

Exam #

Sep 24

L1: Introduction to Cells 

 

Ch. 1

L1.pdf

1

Sep 29

L2: Techniques in Cell Biology

Ch. 8 not sections on NMR, Mass Spec, FRET and genetics (553-566)

Ch. 9 all

L2.pdf

PS1.pdf

PS1KEY.pdf

1

Oct 1

L3: Plasma Membrane Structure and Function I

 

Ch. 3 125-142; 153-159;

Ch. 10

L3.pdf

PS2.pdf

PS2KEY.pdf

1

Oct 6

L4: Plasma Membrane Function II

 

Ch. 11

L4.pdf

PS3.pdf

PS3KEY.pdf

1

Oct 8

L5: Endoplasmic Reticulum and

Golgi Apparatus

 

Ch. 11,

Ch. 12 p695-704 & 723-745

Ch. 6, p388-399

Munro.pdf, Cell V48, p899-907

L5.pdf

PS4.pdf

PS4KEY.pdf

1

Oct 13

L6: Membrane Traffic

 

Ch. 12 p695-704 & 723-745

Ch. 6, p388-399

L6.pdf

PS5.pdf

PS5KEY.pdf

Munro et al., Cell V48, p899-907

 

1

Oct 15

L7: Vesicular Transport

 

Ch. 13

Lec7.pdf

1

Oct 20

Exam 1

Mid1_08practice.pdf

Mid1_08practiceKEY.pdf

 

exam1KEYA.pdf

exam1KEYB.pdf

 

Oct 22

Signal Transduction I

 

Ch. 15

L8.pdf

PS6.pdf

PS6KEY.pdf

2

Oct 27

Signal Transduction II

 

 

Ch. 15

L9.pdf

2

 

 

 

Oct 29

Signal Transduction III/ The Cytoskeleton - Microtubules

Ch. 15/16

L10.pdf

Margolis.pdf

2

Nov 3

L11: The Cytoskeleton – Microtubules

 

Ch. 16

PS7.pdf

PS7KEY.pdf

L11.pdf

2

 

Nov 5

L12: The Cytoskeleton – Microfilaments

 

Ch. 16

PS8.pdf

PS8KEY.pdf

L12.pdf

2

 

Nov 10

Exam 2

Mid2_08practice.pdf

Mid2_08KEY.pdf

Study List.pdf

Exam2AKEY.pdf

Exam2BKEY.pdf

 

 

Nov 12

Cell Division and Cycle Regulation

 

Ch 17

L13.pdf

PS9.pdf

 

 

Nov 17

Cell Cycle Regulation and Cell Death

 

Ch. 17, 18

L14.pdf

PS9KEY.pdf

3

 

Nov 19

L15: The Nucleus

 

Ch. 4

L15.pdf

PS10.pdf

3

 

Nov 24

L16: Cell-Cell Interactions/Extracellular Matrix

 

Ch. 19

 

3

 

Nov 26

HOLIDAY

 

 

 

Dec 1

L17: Cell Biology of Development and Cancer

Ch. 20, 22, 23

Selected reading to be determined

3

 

Dec 3

Review

 

 

3

 

Dec 8

Exam 3          12:00 – 3:00 PM

 

 

 

 

 

Sections

 

There is no requirement to attend section and you may attend any section and as many sections as you like.  In general, the TAs will answer questions and go over problem sets and practice exams.  Exams are distributed in section.

 

Beginning Week of 9/28/09

Sec

Day

Time

Building

Room

TA

A

Mon

2:00PM-3:10PM

Phys Sciences

136

Gary Longo

B

Mon

3:30PM-4:40PM

Phys Sciences

136

Gary Longo

C

Thur

8:30AM-9:40AM

PhysSciences

130

Mikhail Binnewies

D

Fri

9:30AM-10:40AM

PhysSciences

130

Duylinh Nguyen

E

Fri

11:00AM-12:10PM

PhysSciences

130

Duylinh Nguyen

G

Thur

10:00AM-11:10AM

Earth & Marine

B214

Mikhail Binnewies

H

Thur

12:00PM-1:10PM

Earth & Marine

B214

David Ludwig

I

Fri

2:00PM-3:10PM

Eight Acad

252

David Ludwig

 

 

 

 Assistance with Disabilities

Students with disabilities who need special assistance with this course should provide the course coordinator with documentation from the Disabilities Resource Center at the first two meetings of the class. You can make an appointment 9-2089 or email drc@ucsc.edu

 

TA Contact and Office Hours

 

David Ludwig: davidludwig7@gmail.com

 

Thimann 347, Monday 1-2pm and Tuesdays 12:00-1:00 pm

 

Duylinh Nguyen: dlnguyen87@gmail.com

 

            Thimann 347, Monday and Tuesdays at 9-10am

 

Gary Longo: garycharleslongo@gmail.com

 

            Thimann greenhouse, Thursday 11:30-1:30

 

Mikhail Binnewies: mbinnewi@ucsc.edu

 

            Thimann 329, Wednesday 11-12 and Thursday 4-5 Thimann 329

 

Web pages:

 

1)  The inner life of a cell:

 

http://multimedia.mcb.harvard.edu/

 

 

2)  Molecular movies: a portal to cell and molecular animation

 

http://www.molecularmovies.com/showcase/index.html

 

3) Molecular machines

 

http://www.fbs.osaka-u.ac.jp/labs/namba/npn/index.html

 

4) apoptosis

 

http://stke.sciencemag.org/content/vol2007/issue380/images/data/tr1/DC1/Apoptosis_WEHI.mov

 

5) growth cone motility

http://www.jove.com/index/details.stp?ID=662

 

6) rap cell biology

 

http://www.facebook.com/ext/share.php?sid=95839590085&h=J2kHw&u=zbBKt&ref=nf

 

7) cilia and flagella

 

http://www.northland.cc.mn.us/biology/biology1111/animations/flagellum.html

 

8) Cell scales

 

http://learn.genetics.utah.edu/content/begin/cells/scale/

 

Exams and Grading

 

There will be three exams. The first two will each cover the material from one third of the course and each will be worth 100 points. The third and final exam will be comprehensive, covering both the material covered in the final third of the course as well as key material covered in the earlier part of the quarter; it will be worth 150 points. The exam dates and the classes covered on each exam are indicated in the syllabus. The exam format will be multiple choice. You will need to bring the pink, whole page scantron sheets to exams. These are available at the bookstore.

Cheating on exams will not be tolerated.  Evidence of cheating will result in a zero score for that exam and will be reported to the relevant College Provost.

Make-up exams will only be allowed if there is a legitimate reason such as illness, serious family emergency, or a University sponsored event. The make-up will be an oral exam given by me. You are responsible for contacting me BEFORE the exam to notify me of your illness or other excuse. YOU are also responsible for contacting me AFTER the exam to schedule your make-up. The make-up must be scheduled within 48H of the exam.

 

 

Regrading policy

 

Exams are graded by computer. If you have a question about an exam grade, you must talk with me within a week after the exams are handed back.

 

 

 


Biochemistry Review Guide

 

Chapters 2,3 and 4 of your textbook covers many of these concepts.

 

You should be familiar with the following-

 

Different types of chemical bonds and their relative strengths

            Covalent bonds

            Ionic bonds

            Hydrogen bonds

            van der Waals forces

 

Properties of Water

 

Biological Molecules and  their polymers

 

            Sugars and Polysaccharides

                        simple monosaccharides (CH2O)n

                                    glucose, fructose, ribose

                        glycogen, starch

 

            Fatty acids and lipids

                        Basic fatty acid structure

                                    CH3(CH2)nCOOH

                        Basic structure of a triglyceride = glycerol + 3 fatty acid chains

                        Basic structure of phospholipid

 

            Nucleotides and Nucleic Acids

                        Bases - A, C, G, T, U

                        Base + (ribose or deoxyribose) = nucleoside

                        Nucleoside + phosphates = nucleotide

                        purines vs. pyrimidines - be able to draw basic ring structure for each

                        ribo- vs. deoxyribonucleic acid (be able to draw the sugar/phosphate backbone for

each)

 

            Amino Acids and Proteins

                        Know the basic structure of an amino acid

                        Know the names of the 20 amino acids (knowing their 1 letter                                                                       abbreviation is also helpful)

                        For each amino acid you should know which of the folllowing four                                                               categories it belongs in:

                                                non-polar

                                                polar

                                                charged basic

                                                charged acidic

                        Draw a peptide bond

                        Draw a disulfide bridge between cysteines

                        Protein structure

                                    primary structure - amino acid sequence

                                    secondary structure - alpha helices and beta sheets

                                    tertiary structure - longer range interactions leading to domains                                                                      and final folded form of single polypeptide

                                    quaternary structure - interactions between different                                                                                       polypeptides to form a multiprotein complex

                        Understand the importance of hydrophobic interactions in protein                                                                 folding

                        Know how alpha helices and parallel or antiparallel beta sheets work to                                                         mask the polar peptide bonds in a folded protein - be able to                                                                    draw these structures

                        Understand the idea of a domain in a folded protein

                       

Thermodynamics and Reaction Kinetics

            Understand the Gibbs free energy equation and know what each term means

                        DG = DH - TDS

            Reactions with a negative DG value will proceed spontaneously, but they must                                               overcome an activation energy barrier

            Enzymes act as biological catalysts to lower the activation energy for a                                                          reaction.

            Enzymes do not alter the DG of a reaction.

            Reactions with a positive DG will not proceed spontaneously, however they can be coupled

to reactions with a larger negative DG value.

            Many reactions are coupled to hydrolysis of ATP.

                        ATP --> ADP + Pi      DG = -7.3 kcal/mol

The energy from ATP hydrolysis can be coupled to a reaction with a positive                                                DG to allow it to proceed.